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Promega improm-ii reverse transcriptase kit
Improm Ii Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/improm-ii+reverse+transcriptase+kit/improm+ii+reverse+transcription+system/10__1007_slash_s00344___025___11737___0-102-18-22
Average 90 stars, based on 1 article reviews
improm-ii reverse transcriptase kit - by Bioz Stars, 2026-09
90/100 stars

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Synthesized:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

Reverse Transcription:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

cDNA Synthesis:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

Real-time Polymerase Chain Reaction:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

SYBR Green Assay:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

Purification:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

Virus:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

Recombinant:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

RNAscope:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

Multiplex Assay:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

Gene Expression:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

Bacteria:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

Software:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

Microscopy:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

Concentration Assay:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.

Isolation:

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites.
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse tran scriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambda
Article Snippet: ImProm-II TM Reverse Transcriptase Kit , Promega , Cat# A3803.

Article Title: Prenatal antibiotics reduce breast milk IgA and induce dysbiosis in mouse offspring, increasing neonatal susceptibility to bacterial sepsis.
Article Snippet: Real-time PCR reactions were carried out using the Fast Sybr Green PCR kit (QuantiStudio 7 Flex RealTime PCR, Applied Biosystems).

Article Title: Patients with STAT1 Gain-of-function Mutations Display Increased Apoptosis which is Reversed by the JAK Inhibitor Ruxolitinib
Article Snippet: Retrotranscription was performing with 200ng of total RNA using the IMPROM-II Reverse Transcriptase Kit (Promega) in a total volume of 20μL, with the following steps: 25° C for 5 ‘, 42° C for 1 h and 70° C for 15 min.

Article Title: Impact of SARS-CoV-2 on the male reproductive tract: insights from semen analysis and cryopreservation.
Article Snippet: Then, 500 ng RNA was retro-transcribed with ImProm-II Reverse Transcriptase kit (Promega) following manufacturer’s instruction and random primers (0,5 ug/ul). qPCR assay was performed with Luna Universal qPCR Master Mix (New England Biolabs) on 10 ng cDNA template/reaction using exon-spanning primers (final concentration 1 μM).

Article Title: Peromyscus leucopus , Mus musculus , and humans have distinct transcriptomic responses to larval Ixodes scapularis bites
Article Snippet: RNA was subjected to cDNA synthesis using the ImProm-II Reverse transcriptase kit (Promega), and quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad).

Article Title: Composition for preventing or treating neuroinflammatory disease comprising didanosine
Article Snippet: In this regard, RNA was extracted from the isolated hippocampal tissues and used to synthesize cDNA with ImProm-II Reverse Transcriptase kit (Promega).

Article Title: MicroRNAs as Key Regulators of Thermal Adaptation in Coffee Plants: Insights from Genotypic Variations and Potential Applications in Climate Resilience
Article Snippet: Currently, predicted changes to the climate scenario are alarming, and the search for technologies to mitigate the effects of heat stress on crops is a necessity to maintain the global food supply.. Thus, understanding the underlying mechanisms that act in the regulation of elevated temperature stress in large crops such as coffee plants is relevant and may be useful for this purpose, which can be expanded to other plants.. MiRNAs are small molecules that act in the post-transcriptional regulation of their target genes and have already been reported to act in the thermal regulation of many species.



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